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FLC or MCFG were dissolved in distilled water at a concentration of 10 mg/ml, and added to the culture medium, to a final concentration indicated for each single experiment

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Ca 2+ is a master regulator of eryptosis, mediating the Gardos effect-associated RBC dehydration and reduction of cell volume (cell shrinkage), calpain activation-mediated cytoskeleton remodeling (membrane blebbing), and PS externalization, which are major hallmarks of eryptosis

10.1016/j.brainres.2015.09.008 42